Fourier Shell Correlation (FSC) server
Welcome to the PDBe Fourier Shell Correlation (FSC) server!
This web service will calculate and plot the FSC curve from two 3D reconstructions that you upload.
You will be able to download the FSC curve as an XML file that can then be uploaded
as a part of a deposition to EMDB. This server uses the EMAN2
package
for the FSC calculation. The implementation of the 3 sigma and half bit threshold
curves is based on Imagic code kindly provided by Michael Schatz (ImageScience
Software GmbH).
Supported input file formats
include CCP4/MRC, Imagic, Spider,
EMAN2 HDF5 and others. We have one test data sets that you can use to try out the service
here. We appreciate your continuing
support and
feedback!
The uploaded maps will be deleted after the calculation is completed.
Quick links
Recent Entries
(Show all)
Pseudorabies virus nuclear C-capsid (WT) vertices determined in situ
Subtomogram average of pseudorabies virus nuclear egress complex (UL31/34) determined in situ
Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (Substate C)
Structure of the transcription termination factor Rho bound to RNA at the PBS and SBS
Tertiary structure of an individual particle of self-folding RNA polymer (particle #020)
S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP - crown focused map
Central rod disk in C1 symmetry of high-resolution phycobilisome quenched by OCP (local refinement)
focused map of complex I peripheral arm from SC respirasome, murine liver
In situ structure of the Nitrosopumilus maritimus S-layer - Six-fold symmetry (C6)
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 and TSSC4 (Map 5)
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 7.4,200 mM KCl
Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated and neddylated conformation - focused on E2-like density
Pseudorabies virus primary enveloped (perinuclear) C-capsid (US3 KO) vertices determined in situ
Local refinement map of G protein in detergent-solubilized human CaSR-miniGisq complex
Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines (cH125 TTT)
Tertiary structure of an individual particle of self-folding RNA polymer (particle #024)
RNA polymerase II core initially transcribing complex with an ordered RNA of 8 nt
Tertiary structure of an individual particle of self-folding RNA polymer (particle #013)
Cryo-EM structure of the GI.4 Chiba VLP complexed with the CV-1A1 Fv-clasp
Tertiary structure of an individual particle of self-folding RNA polymer (particle #011)
Structure of human calcium-sensing receptor in complex with Gi3 protein in nanodiscs
S. thermodepolymerans KpsM-KpsE in Apo 2 state with rigid body fitted KpsT
Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 2, Map 2)
S. thermodepolymerans KpsM-KpsE in Glycolipid 2 state with rigid body fitted KpsT
Tertiary structure of an individual particle of self-folding RNA polymer (particle #016)
Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines
P-glycoprotein in complex with UIC2 Fab and triple elacridar molecules in nanodisc
P-glycoprotein in complex with UIC2 Fab and triple elacridar molecules in LMNG detergent
CryoEM structure of Nal1 protein, allele SPIKE, from Oryza sativa japonica group
Activated CRAF/MEK heterotetramer from focused refinement of CRAF/MEK/14-3-3 complex
Structure of human calcium-sensing receptor in complex with chimeric Gq (miniGisq) protein in detergent
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 4, Map 4)
S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - KpsT focused map
Cryo-EM structure of human Pannexin-3 R36S/F40R variant in pre-open state
Focused refinement of the 40S subunit head of the 80S Giardia lamblia ribosome at 2.94 angstroms resolution.
TRPM7 structure in complex with anticancer agent CCT128930 in closed state
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 7.4, 5 mM KCl and 135 mM NaCl
Tertiary structure of an individual particle of self-folding RNA polymer (particle #021)
Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 CPH domain
Herpes simplex virus 1 nuclear C-capsid (WT) vertices determined in situ
CryoEM structure of activated CRAF/MEK/14-3-3 complex with NST-628
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in open state
RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state b (composite structure)
Structure of DDM1-nucleosome complex in the ADP-BeFx state with DDM1 bound to SHL2 and SHL-2
Local refinement map of CaSR transmembrane domain (TMD) in detergent-solubilized human CaSR-miniGi1 complex
Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines (H5/1 GCN4)
Tertiary structure of an individual particle of self-folding RNA polymer (particle #026)
S. thermodepolymerans KpsMT-KpsE in Apo 2 state - KpsT focused map
Knockout of GMC-oxidoreductase genes reveals that functional redundancy preserves mimivirus essential functions
Structure of the transcription termination factor Rho in complex with Rof
Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 ARIH-RBR element
Structure of human calcium-sensing receptor in complex with chimeric Gq (miniGisq) protein in nanodiscs
focused map of murine liver complex I peripheral arm in the closed conformation
focused map of murine liver complex I membrane arm in the closed conformation
global refinement of murine liver complex I in the open conformation
focused map of the peripheral arm of complex I from murine liver in the open conformation
focused map of the membrane arm of complex I from murine liver in the open conformation
global refinement of murine brain complex I in the closed conformation
focused map of the peripheral arm of complex I from murine brain in the closed conformation
focused map of the membrane arm of murine brain complex I in the closed conformation
global refinement of complex I from murine brain in the open conformation
focused map of the peripheral arm of murine brain complex I in the open conformation
focused map of the membrane arm of murine brain complex I in the open conformation
Local refinement map of CaSR extracellular domain (ECD) in detergent-solubilized human CaSR-miniGi1 complex
Full-length human cystathionine beta-synthase with C-terminal 6xHis-tag, SAM bound, activated state, local single particle reconstruction
CryoEM structure of nucleotide-free form of the nitrogenase iron protein from A. vinelandii
S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - consensus map
HSV-1 DNA polymerase-processivity factor complex in halted elongation state consensus map
CryoEM structure of human PI3K-alpha (P85/P110-H1047R) with QR-7909 binding at an allosteric site
Central rod disk in D3 symmetry of high-resolution phycobilisome quenched by OCP (local refinement)
Cryo-EM structures of the head region of full-length ERGIC-53 with MCFD2 (form B)
Local refinement map of CaSR transmembrane domain in nanodisc-reconstituted human CaSR-miniGisq complex
Cryo-EM structure of Pyrococcus furiosus transcription elongation complex
Consensus map of HSV-1 DNA polymerase-processivity factor complex in pre-translocation state
Open-state cryo-EM structure of human TRPV3 in presence of 2-APB in cNW30 nanodiscs
Tertiary structure of an individual particle of self-folding RNA polymer (particle #014)
Tertiary structure of an individual particle of self-folding RNA polymer (particle #022)
CryoEM structure of Nal1 protein, allele IR64, from Oryza sativa indica cultivar
Cryo-EM structure of human Elp123 in complex with tRNA, S-ethyl-CoA, 5'-deoxyadenosine and methionine
Local refinement map of CaSR extracellular domain (ECD) in detergent-solubilized human CaSR-miniGisq complex
focused map of complex I peripheral arm from A respirasome, murine liver
Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer
S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - KpsM focused map
Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines. H5 GCN4
Rod from high-resolution phycobilisome quenched by OCP (local refinement)
Cryo-EM structure of human Elp123 in complex with tRNA, desulpho-CoA, 5'-deoxyadenosine and methionine
focused map of complex I membrane arm from A respirasome, murine liver
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 2.5
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 6.0, 200 mM KCl
Local refinement map of CaSR transmembrane domain in nanodisc-reconstituted human CaSR-Gi3 complex
Inactivated-state cryo-EM structure of human TRPV3 in presence of tetrahydrocannabivarin (THCV) in cNW30 nanodiscs
Inactivated-state cryo-EM structure of human TRPV3 in presence of 2-APB in cNW30 nanodiscs
Focused refinement map of BRR2 region of the human minor pre-B complex
Full-length human cystathionine beta-synthase with C-terminal 6xHis-tag, SAM bound, activated state, helical reconstruction
Cryo-EM structure of human Elp123 in complex with tRNA, acetyl-CoA, 5'-deoxyadenosine and methionine
S. thermodepolymerans KpsM-KpsE in Glycolipid 1 state with rigid body fitted KpsT
Top cylinder bound to OCP from high-resolution phycobilisome quenched by OCP (local refinement)
Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (Substate B)
Inner channel lipids regulated gating mechanism of human pannexins
Herpes simplex virus 1 cytosolic C-capsid (WT) vertices determined in situ
Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated conformation - symmetry expanded unneddylated dimer
Structure of human calcium-sensing receptor in complex with Gi1 (miniGi1) protein in detergent
focused map of complex I membrane arm from C respirasome, murine liver
Tertiary structure of an individual particle of self-folding RNA polymer (particle #018)
Full-length human cystathionine beta-synthase with C-terminal 6xHis-tag, basal state, single particle reconstruction
Local refinement map of CaSR extracellular domain in nanodisc-reconstituted human CaSR-miniGis complex
Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (form A)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 5.5
Tertiary structure of an individual particle of self-folding RNA polymer (particle #030)
Subtomogram average of pseudorabies virus nuclear egress complex helical form (UL31/34) determined in situ
Tertiary structure of an individual particle of self-folding RNA polymer (particle #023)
S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - consensus map
focused map of the canonical CIV from SC respirasome, murine liver
Tertiary structure of an individual particle of self-folding RNA polymer (particle #015)
Full-length human cystathionine beta-synthase, basal state, single particle reconstruction
Local refinement map of G protein in nanodisc-reconstituted human CaSR-miniGis complex
Open-state cryo-EM structure of human TRPV3 in presence of tetrahydrocannabivarin (THCV) in cNW30 nanodiscs
Full-length human cystathionine beta-synthase with C-terminal 6xHis-tag, basal state, helical reconstruction
Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (Substate D)
Bottom cylinder of high-resolution phycobilisome quenched by OCP (local refinement)
80S Giardia lamblia ribosome at 2.66 angstroms resolution with Emetine in the the V1 conformation
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in closed state
focused map of the SCAF1-containing complex IV from SC respirasome, murine livers
Human TWIK-related acid-sensitive potassium channel TASK3 at pH 6.0, 5 mM KCl and 135 mM NaCl
Full-length human cystathionine beta-synthase with C-terminal 6xHis-tag, SAM bound, activated state, local helical reconstruction
Structure of the transcription termination factor Rho in complex with Rof and ADP
Cryo-EM structure of human Elp123 in complex with 5'-deoxyadenosine and methionine
Tertiary structure of an individual particle of self-folding RNA polymer (particle #029)
Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in intermediate state
Local refinement map of G protein in nanodisc-reconstituted human CaSR-miniGisq complex
focused map of complex I membrane arm from SC respirasome, murine liver
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 and TSSC4 (Map 6)
Local refinement map of G protein in nanodisc-reconstituted human CaSR-Gi3 complex
Tertiary structure of an individual particle of self-folding RNA polymer (particle #019)
RNA polymerase II core initially transcribing complex with an ordered RNA of 10 nt
EM map of de novo designed protein nanopore RNR_C6_3 using a reinforcement learning approach
Pseudorabies virus cytosolic C-capsid (US3 KO) vertices determined in situ
focused map of complex I peripheral arm from C respirasome, murine liver
Full-length human cystathionine beta-synthase, basal state, partially degraded tetramer
Local refinement map of CaSR extracellular domain in nanodisc-reconstituted human CaSR-Gi3 complex
Tertiary structure of an individual particle of self-folding RNA polymer (particle #027)
Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 1, Map 1)
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 3, Map 3)
Cryo-EM structures of the head region of full-length ERGIC-53 with MCFD2 (Substate A)
Local refinement map of CaSR transmembrane domain (TMD) in detergent-solubilized human CaSR-miniGisq complex
Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer (composite map)
Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated and neddylated conformation - focused cullin dimer
Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 DOC domain
Map of YPEL5-bound WDR26 dimer obtained by focused refinement of the WDR26-CTLH subcomplex
Full-length human cystathionine beta-synthase, basal state, helical reconstruction
Tertiary structure of an individual particle of self-folding RNA polymer (particle #028)
Pseudorabies virus cytosolic C-capsid (WT) vertices determined in situ
S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 1 - KpsM focused map
Knockout of GMC-oxidoreductase genes reveals that functional redundancy preserves mimivirus essential functions
Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines (H2/1 GCN4)
Structure of CUL9-RBX1 ubiquitin E3 ligase complex - hexameric assembly
In situ structure of the Nitrosopumilus maritimus S-layer - Composite map between C2 and C6
Local refinement map of CaSR extracellular domain in nanodisc-reconstituted human CaSR-miniGisq complex
Tertiary structure of an individual particle of self-folding RNA polymer (particle #025)
Tertiary structure of an individual particle of self-folding RNA polymer (particle #017)
Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 ARM9 domain
Pseudorabies nuclear C-capsids (US3 KO) vertices determined in situ
Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated and neddylated conformation - focused dimeric core
Umb1 umbrella toxin particle (local refinement of UmbB1 bound ALF of UmbC1 and UmbA1)
Synechocystis PCC 6803 Phycobilisome quenched by OCP, high resolution
Consensus map of HSV-1 DNA polymerase-processivity factor complex in exonuclease state
Tertiary structure of an individual particle of self-folding RNA polymer (particle #012)
CryoEM structure of human PI3K-alpha (P85/P110-H1047R) with QR-8557 binding at an allosteric site
Local refinement map of CaSR transmembrane domain in nanodisc-reconstituted human CaSR-miniGis complex
Structure of human calcium-sensing receptor in complex with chimeric Gs (miniGis) protein in nanodiscs
S. thermodepolymerans KpsMT-KpsE with bound glycolipid - state 2 - KpsT focused map
S. thermodepolymerans KpsMT(E151Q)-KpsE in complex with ATP - consensus map
RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state a (composite structure)
Local refinement map of G protein in detergent-solubilized human CaSR-miniGi1 complex
